Journal: STAR Protocols

Article Title: Simplified mass cytometry protocol for in-plate staining, barcoding, and cryopreservation of human PBMC samples in clinical trials

doi: 10.1016/j.xpro.2022.101362

Figure Lengend Snippet:

Article Snippet: Anti-Ki-67 (B56)-168Er—100 Tests (1:100) , Fluidigm , Cat#3168007B.

Techniques: Recombinant, Saline, Staining, Blocking Assay, Isolation, Software, Cell Counting, Cytometry

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood

doi: 10.1007/978-1-0716-2553-8_10

Figure Lengend Snippet: Whole blood phosphoflow panel 1

Article Snippet: 172 Yb , Anti-Ki-67 , B56 , Fluidigm 3172024B.

Techniques:

Journal: Frontiers in Pediatrics

Article Title: Neurogenesis Is Reduced at 48 h in the Subventricular Zone Independent of Cell Death in a Piglet Model of Perinatal Hypoxia-Ischemia

doi: 10.3389/fped.2022.793189

Figure Lengend Snippet: Fluorescent microphotographs of Ki67 [cell proliferation, (A,B) ], Sox2/Ki67 [neural stem/progenitor cell proliferation, (C,D) ], and DAPI [nuclei, (E,F) ] immune-stained samples from control and HI piglets. Ki67 positive cells (green) are abundant in the control piglet (A) , whereas HI (B) significantly reduced its counts by half in L-SVZ and DL-SVZ (both *** p < 0.001). Double Sox2 (red) + /Ki67 (green) + cells are observed in control animals; again, HI reduced neural stem/progenitor cell counts in L-SVZ and DL-SVZ (both *** p < 0.001). DAPI immunostaining reveals total nuclei. LV, lateral ventricle; SVZ, subventricular zone; CDT, caudate nucleus; PvWM, periventricular white matter. Original magnification 400×. Scale bar: 100 μm.

Article Snippet: For single labeling, radial-glia/neural stem cells were identified using an anti-glial fibrillary acidic protein (GFAP) antibody to assess neurogenic activity close to the ventricular wall (mouse anti-GFAP, 1:100, MA5-12023, Thermo Fisher, United States); an anti-doublecortin (DCX) antibody was used to identify young neurons/neuroblasts (mouse anti-DCX, 1:50, sc-271390, Santa Cruz Biotechnology, United States); cell proliferation was identified by an anti-Ki67 antibody (mouse anti-Ki67; 1:50, STJ96966, St Johns Labs, United Kingdom).

Techniques: Staining, Control, Immunostaining

Journal: Cancer cell

Article Title: Dendritic cell paucity leads to dysfunctional immune surveillance in pancreatic cancer

doi: 10.1016/j.ccell.2020.02.008

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: anti-human Ki-67 (B56) , Fluidigm , #3161007B.

Techniques: Recombinant, Cell Stimulation, Enzyme-linked Immunosorbent Assay, CyQUANT Assay, LDH Cytotoxicity Assay, Expressing, Staining, Derivative Assay, Software

Journal: Cell reports

Article Title: Single-cell analysis of the human pancreas in type 2 diabetes using multi-spectral imaging mass cytometry

doi: 10.1016/j.celrep.2021.109919

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Article Snippet: 168Er-Ki67 (Clone B56) , Fluidigm , Cat#3168022D.

Techniques: Software

Journal: STAR Protocols

Article Title: CyTOF protocol for immune monitoring of solid tumors from mouse models

doi: 10.1016/j.xpro.2022.101949

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Article Snippet: Anti-Mouse KI67-162Dy dilution 1:200 , Standard BioTools , 3162012B.

Techniques: Blocking Assay, Purification, Recombinant, Staining, Sterility, Antibody Labeling, Software, Transferring, Microscopy

Journal: Oncology Letters

Article Title: SOX2-positive upper tract urothelial carcinoma: Clinicopathological characteristics and therapeutic implications

doi: 10.3892/ol.2026.15629

Figure Lengend Snippet: Immunohistochemical staining and molecular marker expression in UTUC. (A) Immunohistochemical staining patterns for molecular subtype markers (CK5/6, CK14, Uroplakin 2, CK20 and GATA3). Scale bar, 200 µm. (B) Immunohistochemical expression of PD-L1 and ADC targets (Nectin-4 and TROP-2) in UTUC tissue. (a) Negative PD-L1 expression. (b) Positive PD-L1 expression. Scale bar, 100 µm. (c) Negative Nectin-4 expression (intensity, 0). (d) Weak Nectin-4 expression (intensity, 1+). (e) Moderate Nectin-4 expression (intensity, 2+). (f) Negative TROP-2 expression. (g) Positive TROP-2 expression. Scale bar, 200 µm. UTUC, upper tract urothelial carcinoma; PD-L1, programmed cell death ligand 1; ADC, antibody-drug conjugate; TROP-2, trophoblast cell surface antigen 2; CK, cytokeratin.

Article Snippet: Molecular subtypes were evaluated using antibodies against CK5/6 (418081, 1:1; Nichirei Biosciences, Inc.), CK20 (413491, 1:1; Nichirei Biosciences, Inc.), GATA3 (418201, 1:1; Nichirei Biosciences, Inc.), UPK2 (418121, 1:1; Nichirei Biosciences, Inc.) and CK14 (NCL-L-LL002, Leica, 1:50; Leica Biosystems; ) ( , ).

Techniques: Immunohistochemical staining, Staining, Marker, Expressing